Detection of Ofloxacin Resistance by Nitrate Reductase Assay in Mycobacterium tuberculosis Isolates from Extrapulmonary Tuberculosis

Shrivastava, N. and Set, R. and Bankar, S. and Shastri, J. (2017) Detection of Ofloxacin Resistance by Nitrate Reductase Assay in Mycobacterium tuberculosis Isolates from Extrapulmonary Tuberculosis Indian J Med Microbiol, 35 (1). pp. 69-73.

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Abstract

Context: Increased use of fluoroquinolones to treat community‑acquired infections has led to the decreased susceptibility to Mycobacterium tuberculosis. There is a paucity of data on ofloxacin (OFX) resistance detection by nitrate reductase assay (NRA). Hence, the present study was carried out to find the efficacy of NRA for detection of OFX resistance in M. tuberculosis isolated from extrapulmonary tuberculosis (EPTB) cases. Aims: (1) To compare sensitivity, specificity and median time required to obtain results by NRA with economic variant proportion method (PM) for detection of OFX resistance.(2) To determine the extent of OFX resistance in clinical isolates of M. tuberculosis. Settings and Design: Seventy‑three M. tuberculosis isolates from cases of EPTB were subjected to economic variant of PM for isoniazid, rifampicin and OFX. NRA was done for detection of OFX resistance. Subjects and Methods: Seventy‑three isolates from clinical samples of suspected EPTB received in the Department of Microbiology were included in the study. Drug susceptibility test was performed on Lowenstein– Jensen medium with and without drugs. Statistical Analysis Used: Of turnaround time was done by Mann–Whitney test on SPSS (version 19, released in 2010, IBM Corp, Armonk NY), P < 0.05. Results: OFX resistance was seen in nine isolates. The sensitivity and specificity of OFX resistance by NRA was 100% and 96.87%, respectively. Median time required to obtain results by NRA was 10 days as compared to 28 days by PM. Conclusions: NRA is a specific and sensitive method for detection of OFX resistance in resource‑restricted settings.

Item Type: Article
Uncontrolled Keywords: Multidrug‑resistant tuberculosis; Mycobacterium tuberculosis; Pre‑XDR; Proportion method
Subjects: Tuberculosis > Extra Pulmonary TB
Tuberculosis > Laboratory
Tuberculosis > Drug Resistant TB
Institutions: National Task Force, Zonal Task Force & State Task Force of Medical Colleges
Depositing User: Mr SANJAY SINGH
Date Deposited: 11 Apr 2017 21:06
Last Modified: 11 Apr 2017 21:06
URI: http://tbresearch.ntiindia.org.in/id/eprint/2265

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