Performance of a pyrosequencing platform in diagnosing drugresistant extra-pulmonary tuberculosis in India

Georghiou, S. B. and Ajbani, K. and Rodrigues, C. and Rodwell, T. C. (2016) Performance of a pyrosequencing platform in diagnosing drugresistant extra-pulmonary tuberculosis in India Int J Tuberc Lung Dis, 20 (2). pp. 160-165.

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S E T T ING: Pyrosequencing diagnostic assays have shown great utility in identifying and characterizing pulmonary drug-resistant tuberculosis (TB) infections. However, the method has yet to be evaluated for the diagnosis of drug-resistant extra-pulmonary TB (EPTB). OBJECTIVE : To evaluate the performance of a pyrosequencing platform in establishing molecular drug resistance profiles for 79 clinical EPTB specimens at a referral center for drug-resistant TB in India. DESIGN: Genotypic drug resistance profiles were established for all 79 non-pulmonary, culture-positive TB clinical specimens. Acid-fast bacilli smear microscopy, MGITe 960e culture and drug susceptibility testing were performed on all specimens for reference. RESULT S : In comparison to MGIT 960, the sensitivity and specificity of pyrosequencing in detecting drug resistance among specimens was found to be respectively 100% and 100%, 67% and 98%, and 100% and 100% for isoniazid, rifampicin, and the fluoroquinolones. No EPTB specimens were phenotypically resistant to any of the injectables, but the specificity of the assay was determined to be 100%, 98%, and 98% for amikacin, kanamycin, and capreomycin. CONCLUS IONS : Pyrosequencing is a rapid, appropriate technology for the diagnosis of isoniazid-, fluoroquinolone-, and potentially injectable drug-resistant EPTB clinical specimens, and should be considered as an alternative to conventional growth-based diagnostic methods for EPTB when resistance to these drugs is suspected.

Item Type: Article
Uncontrolled Keywords: Molecular diagnostics; Genetic mutations; Drug resistance
Subjects: Tuberculosis > Extra Pulmonary TB
Tuberculosis > Drug Resistant TB
Institutions: National Task Force, Zonal Task Force & State Task Force of Medical Colleges
Depositing User: Mr SANJAY SINGH
Date Deposited: 29 Jan 2016 10:12
Last Modified: 29 Jan 2016 10:12

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