T-h-2 immunity and CD3+ CD45RBlow-activated T cells in mice immunized with recombinant bacillus Calmette-Guerin expressing HIV-1 principal neutralizing determinant epitope

Aravindhan, V. and Narayanan, S. and Gautham, N. and Prasad, V. and Kannan, P. and Jacobs, W. R. Jr. and Narayanan, P. R. (2006) T-h-2 immunity and CD3+ CD45RBlow-activated T cells in mice immunized with recombinant bacillus Calmette-Guerin expressing HIV-1 principal neutralizing determinant epitope FEMS Immunol Med Microbiol, 47. pp. 45-55.

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Abstract

The genetic engineering of Mycobacterium bovis –bacillus Calmette–Guerin to express foreign epitopes is an attractive strategy in the field of epitope vaccines. We constructed an ‘epitope-trap vector' with Mycobacterium tuberculosis chaperonin-10 as a carrier antigen and used it to express the HIV-1 principal neutralizing determinant epitope. We also identified a new chaperonin-10 promoter that was hyperexpressive compared with the heat shock protein-65 promoter. Splenocytes from recombinant bacillus Calmette–Guerin-immunized mice showed enhanced lymphocyte proliferation and interleukin-4 (but not interferon- g ) secretion. The recombinant bacillus Calmette–Guerin-immunized group also exhibited mild delayed-type hypersensitivity reaction and a high frequency of CD3 + CD45RB low -activated T cells, together with high titer of antiprincipal neutralizing determinant immunoglobulin G antibodies. Thus, this epitope delivery system induced strong epitope-specific T-h-2 polarization.

Item Type: Article
Uncontrolled Keywords: Recombinant bacillus Calmette–Gue; Rin (r-BCG); Epitope-trap vector; Chaperonin-10 (Cpn10) promoter; HIV-1 principal neutralizing determinant (PND); Cryptic epitopes
Subjects: Tuberculosis > Laboratory
Institutions: National Institute for Research in Tuberculosis (NIRT), Chennai
Depositing User: ravindra s
Date Deposited: 23 Jul 2013 09:06
Last Modified: 23 Jul 2013 09:06
URI: http://tbresearch.ntiindia.org.in/id/eprint/1500

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