Identification & differentiation of Mycobacterium avium & M. intracellulare by PCR- RFLP assay using the groES gene

Aravindhan, V. and Sulochana, S. and Narayanan, S. and Paramasivan, C. N. and Narayanan, P. R. (2007) Identification & differentiation of Mycobacterium avium & M. intracellulare by PCR- RFLP assay using the groES gene Indian J Med Res, 126. pp. 575-579.

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Abstract

Background & objectives : We report a new polymerase chain reaction (PCR) – restriction fragment length polymorphism (RFLP) assay using mycobacterial groES as a target to identify Mycobacterium avium and M. intracellulare in clinical samples. Methods : The assay was standardized using M. avium and M. intracellulare standard strains obtained from ATCC and was tested with 45 M. avium - M. intracellulare complex (MAC) clinical isolates (Of which 31 were from HIV + individuals). The standard and clinical strains were typed with HPLC based mycolic acid fingerprinting. Results : Three polymorphisms ( BamHI, BstNI and HgaI ) were identified for inter-species differentiation among standard strains; of which, only HgaI was found to be useful in clinical isolates. Of the 45 isolates, 25 were M. avium and 20 were M. intracelluare . MAC isolates, which could not be differentiated by HPLC analysis, were also typed by this method. Interpretation & conclusions : The use of mycobacterial groES as a PCR-RFLP target for M. avium and M. intracellulare is a simple and rapid method that can complement HPLC in their differentiation.

Item Type: Article
Uncontrolled Keywords: GroES; HPLC; MAC; Mycobacterium avium; M. intracellulare; PCR; RFLP
Subjects: Tuberculosis > Laboratory
Institutions: National Institute for Research in Tuberculosis (NIRT), Chennai
Depositing User: S Nandish Prasad
Date Deposited: 21 Jun 2013 09:37
Last Modified: 21 Jun 2013 09:37
URI: http://tbresearch.ntiindia.org.in/id/eprint/1226

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